booltБюллетень Государственного Никитского ботанического садаBulletin of the State Nikitsky Botanical Gardens0513-1634Federal State Funded Institution of Science “The Labour Red Banner Order Nikitsky Botanical Gardens – National scientific Center of the RAS”boolt-1015Research ArticleЗАЩИТА РАСТЕНИЙPLANT PROTECTIONРазработка и применение инновационного олигонуклеотидного инсектицида явол-11 для контроля Ceroplastes japonicus green (подотряд sternorrhyncha)Development and application of innovative oligonucleotide insecticide YAVOL-11 for control of Ceroplastes japonicas Green (suborder Sternorrhyncha)ПлугатарьЮ. В.PlugatarYu. V.

Юрий Владимирович Плугатарь

298648, Республика Крым, г. Ялта, пгт. Никита, спуск Никитский, 52

pcr.product@gmail.comГальчинскийН. В.Gal’chinskyN. V.

Никита Витальевич Гальчинский

295007, Республика Крым, г. Симферополь, проспект Академика Вернадского, 4

ЯцковаЕ. В.YatskovaE. V.

Екатерина Васильевна Яцкова

298648, Республика Крым, г. Ялта, пгт. Никита, спуск Никитский, 52

ШармагийА. К.SharmagiyA. K.

Александр Константинович Шармагий

298648, Республика Крым, г. Ялта, пгт. Никита, спуск Никитский, 52

ОберемокВ. В.OberemokV. V.

Владимир Владимирович Оберемок

298648, Республика Крым, г. Ялта, пгт. Никита, спуск Никитский, 52;

295007, Республика Крым, г. Симферополь, проспект Академика Вернадского, 4

Никитский ботанический сад – Национальный научный центр РАНRussian FederationФГАОУ ВО «КФУ им. В.И. Вернадского»Russian FederationНикитский ботанический сад – Национальный научный центр РАН; ФГАОУ ВО «КФУ им. В.И. Вернадского»Russian Federation2025161020250156717Copyright © Плугатарь Ю.В., Гальчинский Н.В., Яцкова Е.В., Шармагий А.К., Оберемок В.В., 20252025Плугатарь Ю.В., Гальчинский Н.В., Яцкова Е.В., Шармагий А.К., Оберемок В.В.Plugatar Y.V., Gal’chinsky N.V., Yatskova E.V., Sharmagiy A.K., Oberemok V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://boolt.elpub.ru/jour/article/view/1015

Изобретенные в 2008 г. контактные олигонуклеотидные инсектициды (олинциды или ДНК-инсектициды) на основе платформы контактно вводимой антисмысловой ДНК-биотехнологии (КВАДб) были существенно улучшены и переосмыслены. КВАДб (или метод "генетической застёжки-молнии") объединяет молекулярную генетику, биоинформатику и синтез нуклеиновых кислот in vitro. Главным сдвигом парадигмы стала демонстрация того, что немодифицированная антисмысловая ДНК может действовать как контактный инсектицид. Ключевые прорывы включали идентификацию удобных целевых генов (генов рРНК), механизма действия (ДНК-сдерживание) и обнаружение насекомых-вредителей из подотряда грудохоботных (Sternorrhyncha) с высокой восприимчивостью к олинцидам. Данный класс инсектицидов нового поколения создает возможности для разработки инсектицидов, предназначенных для отдельных популяций насекомых-вредителей. В данной работе, проведенной на личинках Ceroplastes japonicus Green в открытом грунте, показан высокий потенциал использования олинцида ЯВОЛ-11 (5′-CGACCGACGAA-3′) в борьбе с ложнощитовкой и их потенциал в качестве замены неселективным фосфоорганическим инсектицидам.

Contact oligonucleotide insecticides (olinscides or DNA insecticides) based on the contact unmodified antisense DNA biotechnology (CUADb) platform, invented in 2008, have been significantly improved and rethought. CUADb (or the "genetic zipper" method) combines molecular genetics, bioinformatics and in vitro nucleic acid synthesis. A major paradigm shift was the demonstration that unmodified antisense DNA can act as a contact insecticide. Key breakthroughs included the identification of convenient target genes (rRNA genes), the mechanism of action (DNA containment) and the detection of sternorrhynchans insect pests highly susceptible to olinscides. This class of new generation insecticides creates opportunities for the development of insecticides intended for individual populations of insect pests. In this work, conducted on Ceroplastes japonicus Green larvae in open ground, the high potential of using olinscide YAVOL-11 (5'-CGACCGACGAA-3') in the fight against false scale insects and their potential as a replacement for non-selective organophosphorus insecticides is demonstrated.

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The authors declare that there are no conflicts of interest present.