Development of biotechnology of virus elimination in micropropagated Malus Sp. and obtaining virus-free planting stocks
https://doi.org/10.36305/0513-1634-2022-144-105-113
Abstract
Chemotherapy with ribavirin has been used as a method for healthy in vitro apple shoots obtaining. Three subcultures of in vitro plants for 45 days each on Murashige and Skoog medium with 50 mg/L of ribavirin resulted in virus elimination with 100% efficiency. Half concentration of Murashige and Skoog mineral components with 0.25 mg/L indolylbutyric acid, 1.25 g/L gelrite, 4 g/L agar, pH 5.7 was efficient for in vitro root formation (50-90%). At the first stage, for the adaptation of apple plants (85-90%) at the soil substrate, after transferring shoots from in vitro conditions, it is necessary to cover them with a plastic cap to retain moisture. Uniform ventilation of the planting stocks is required to prevent the formation of rot and mold.
About the Authors
N. V. Romadanova
РГП «Институт биологии и биотехнологии растений» КН МОН РК; Казахский Национальный Университет. им. аль-Фараби
Russian Federation
Russian Federation
A. B. Tolegen
РГП «Институт биологии и биотехнологии растений» КН МОН РК; Казахский Национальный Университет. им. аль-Фараби
Russian Federation
Russian Federation
S. V. Kushnarenko
РГП «Институт биологии и биотехнологии растений» КН МОН РК
Russian Federation
Russian Federation
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Review
For citations:
Romadanova N.V., Tolegen A.B., Kushnarenko S.V. Development of biotechnology of virus elimination in micropropagated Malus Sp. and obtaining virus-free planting stocks. Bulletin of the State Nikitsky Botanical Gardens. 2022;(144):105-113. (In Russ.) https://doi.org/10.36305/0513-1634-2022-144-105-113
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