Analysis of variability of mitochondrial gene COI and diagnostics of Lycorma delicatula (White, 1845) (Hemiptera, Fulgoroidea, Fulgoridae)
https://doi.org/10.36305/0513-1634-2022-145-178-184
Abstract
Lycorma delicatula is an invasive hemipteran insect species whose harmfulness is observed in the secondary area. The distribution of this species occurs mainly with the preimaginal stages, which are not identified by morphological methods. The use of DNA-barcoding on a fragment of the mitochondrial COI gene for the diagnosis of this species is limited by the lack of an assessment of its intra-specific and inter-specific variability. According to the results of the analysis of the sequences of this molecular marker, Lycorma delicatula well differentiates from other species within the family Fulgoridae and the genus Lycorma (bootstrap values≥85%). The average value of genetic distances according to the Kimura two-parameter model for Lycorma delicatula specimens from the primary area was 1.2%, varying in the range of 0-3.8%. Lycorma delicatula is characterized by the presence of a threshold between intra-specific and inter-specific variability, which is 5.0%. The results are of practical importance for the phytosanitary laboratories.
About the Authors
M. K. Mironova
Федеральное государственное бюджетное учреждение «Всероссийский центр карантина растений»
Russian Federation
Russian Federation
A. V. Shipulin
Федеральное государственное бюджетное учреждение «Всероссийский центр карантина растений»
Russian Federation
Russian Federation
I. O. Kamayev
Федеральное государственное бюджетное учреждение «Всероссийский центр карантина растений»
Russian Federation
Russian Federation
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Review
For citations:
Mironova M.K., Shipulin A.V., Kamayev I.O. Analysis of variability of mitochondrial gene COI and diagnostics of Lycorma delicatula (White, 1845) (Hemiptera, Fulgoroidea, Fulgoridae). Bulletin of the State Nikitsky Botanical Gardens. 2022;(145):178-184. (In Russ.) https://doi.org/10.36305/0513-1634-2022-145-178-184
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