The effect of growth regulators on the effectiveness of micropropagation of strawberries

For the first time, a protocol of microclonal propagation for garden strawberries of ‘Fresca’ cv. from seeds has been developed, including introduction to culture, reproduction and rooting. The object of the study is a strawberry of the garden cultivar ‘Fresca’. The objective of the study was to study the effect of growth regulators and activated carbon on microclonal propagation of the above-mentioned garden strawberry cultivar. The seeds were sterilized with a 0.1% solution of silver nitrate, then washed with a solution of sodium chloride, then washed three times with sterile distilled water. For seed germination, a nutrient medium was used according to the Murashige and Skoog recipe without growth regulators (MS⁰ ). To study the effect of growth regulators on plant development, MS nutrient media with the addition of 6-benzylaminopurine (MS+0.5 mg/l 6-BAP; MS+1.0 mg/l 6-BAP) and thidiazuron (MS+0.5 mg/l TDZ) were used. MS⁰ was used as a control. Culture vessels were placed under cool white fluorescent light, photoperiod 16/8 h light/dark, temperature 23 ± 2 °C. To germinate seeds, it is enough to use the Murashige and Skoog nutrient medium without growth regulators. The effectiveness of seed sterilization with 0.1% silver nitrate solution was quite high and amounted to 97.5%. However, the quality of commercial seeds was low and we received only 3.9% of viable seedlings. Further, the influence of the above concentrations of growth regulators on the regeneration potential of seedlings was studied, using a component composition according to Murashige and Skoog as a basal medium. The limiting factors in the microclonal reproduction of this strawberry cultivar are growth regulators and their concentrations. It is shown that the use of thidiazuron leads to tissue necrosis and complete death of microplants, both during the cultivation of seedlings and when transplanting whole plants to this medium. And 6-benzylaminopurine has a positive effect on the effectiveness of micro-propagation of strawberries of the garden cultivar ‘Fresca’. In our case, 6- benzylaminopurine led to an increase in the multiplication factor and the induction of rhizogenesis (MS+0.5 mg/l 6-BAP). It should be noted that the addition of activated carbon to the nutrient medium stimulated the growth of microplants, but at the same time the multiplication factor decreased (MS+1 mg/l 6-BAP). The formation of the root system was recorded in all clones that were placed on a Murashige and Skoog basal medium and containing 6-benzylaminopurine.

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