Introduction, micropropagation and preservation features of two common fig cultivars in vitro
Abstract
Methods for obtaining an aseptic culture of common fig (Ficus carica L.) cultivars Limonno-Zheltyiy and Nairannejshiy Fioletovyiy under in vitro conditions have been developed. The optimal concentrations of sterilizing agents, exposure time and order of their application were established: 1 min in 70% ethanol, 15 min in 1% Thimerosal solution and 18 min in DezTab solution, which allowed to obtain 90.0% viable explants. The peculiarities of the studied fig cultivars development at the introduction, multiple shoot formation and preservation in vitro stages on Murashige and Skoog (1962) culture medium with different concentrations and combinations of plant growth regulators are revealed. The efficiency of the MS culture medium supplemented with growth regulators 6-BAP at a concentration of 1.5 mg/l, NAA – 0.1 mg/l and GA3 – 0.5 mg/l using for the introduction and propagation of fig explants has been shown. At the same time, the multiplication rate reached 8.1 and 15.4 in Limonno-Zheltyiy and Nairannejshiy Fioletovyiy cultivars, respectively. The method of long-term in vitro preservation of two studied F. carica cultivars for 12 months by slow-growth storage has been optimized. The optimal temperature (8 °C) has been established, at which the growth kinetics decreases and the viability of explants is preserved.
About the Authors
S. V. Chelombit
Никитский ботанический сад – Национальный научный центр РАН
Russian Federation
Russian Federation
N. P. Lesnikova-Sedoshenko
Никитский ботанический сад – Национальный научный центр РАН
Russian Federation
Russian Federation
I. V. Mitrofanova
Главный ботанический сад им. Н.В. Цицина РАН
Russian Federation
Russian Federation
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Review
For citations:
Chelombit S.V., Lesnikova-Sedoshenko N.P., Mitrofanova I.V. Introduction, micropropagation and preservation features of two common fig cultivars in vitro. Bulletin of the State Nikitsky Botanical Gardens. 2024;(151):52-61. (In Russ.)
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