Features of in vitro morphogenesis in lavandin (Lavandula × intermedia Emeric ex Loisel.)
Abstract
Lavandin (Lavandula × intermedia Emerice ex Loiseleur) is a member of the Lamiaceae family and is an aromatic valuable perennial plant. It is highly valued for the resulting essential oil, which is used in perfumery, cosmetics and the pharmaceutical industry. These essential oil plants can be propagated vegetatively, but the low rooting ability of stem cuttings prevents their wide use. The use of biotechnology methods, such as plant tissue and organ culture, makes it possible to in vitro propagation and preservation valuable genotypes. Our study presents the results of in vitro morphogenetic capacity investigation in three lavandin cultivars (ʹRabatʹ, ʹSnezhnyi Barsʹ, ʹTempʹ). The optimal method of sterilization of the initial explants of the studied cultivars was determined: one minute in 70 % ethanol, 12 minutes in 1 % Thimerosal solution and 15 minutes in DezTab solution, which made it possible to obtain up to 90.2% of viable explants. Features of the induction of explant morphogenesis on the MS culture medium supplemented with cytokinins (BAP or kinetin) and the auxin NAA were revealed. A high ability for adventitious shoot formation on a medium with 1.5 mg/l kinetin and 0.05 mg/l NAA has been shown. It was established that lavandin ʹRabatʹ had a higher in vitro morphogenetic capacity compared to the ʹSnezhnyi Barsʹ and ʹTempʹ cultivars.
About the Authors
I. V. Zhdanova
Никитский ботанический сад – Национальный научный центр РАН
Russian Federation
Russian Federation
N. P. Lesnikova-Sedoshenko
Никитский ботанический сад – Национальный научный центр РАН
Russian Federation
Russian Federation
I. V. Mitrofanova
Главный ботанический сад им. Н.В. Цицина РАН
Russian Federation
Russian Federation
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Review
For citations:
Zhdanova I.V., Lesnikova-Sedoshenko N.P., Mitrofanova I.V. Features of in vitro morphogenesis in lavandin (Lavandula × intermedia Emeric ex Loisel.). Bulletin of the State Nikitsky Botanical Gardens. 2024;1(153):61-69. (In Russ.)
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