Phenomenon of cytoplasmic male sterility in sunflower and possible causes of reduced seed setting in F2 hybrids
Abstract
The phenomenon of CMS is widely used in breeding for heterosis for controlled pollination in the production of hybrid seeds. For this purpose, Helianthus annuus L. sterile lines based on the CMS PET1 genotype and fertility restorers carrying the dominant Rf1 allele are used. The nature of variability in pollen fertility in plants of F2 and F3 hybrids obtained from crosses between the VIR 116A line (CMS PET1) and the fertility restorer, VIR 195 was studied. The degree of pollen deficiency (DPD), calculated as the ratio of defective pollen grains to the total number of counted ones (in %), was also estimated, as well as the variability of this indicator within splitting populations. The degree of pollen defectiveness (DPD) was calculated as the ratio of defective pollen grains to the total number of counted ones (in %) and the variability of this indicator in splitting populations was estimated. It was found that plants belonging to phenotypic classes "fertile" can have different DPD indicators. Significant heterogeneity in the F2 population based on pollen quality with a tendency to equalize indicators in F3 was revealed. A decrease in pollen quality was noted in F2 segregants possessing the maternal variant of the STS marker of gene alleles at the Pl5/Pl8 locus. The DPD of plants is most likely determined by the genotype of the plant (sporophyte), and not by the allelic composition of pollen genes (male gametophyte).
About the Authors
O. N. Voronova
Ботанический институт им. В.Л. Комарова Российской академии наук
Russian Federation
Russian Federation
Yu. I. Karabitsyna
Всероссийский институт генетических ресурсов растений имени Н.И. Вавилова
Russian Federation
Russian Federation
N. V. Alpatieva
Всероссийский институт генетических ресурсов растений имени Н.И. Вавилова
Russian Federation
Russian Federation
V. A. Gavrilova
Всероссийский институт генетических ресурсов растений имени Н.И. Вавилова
Russian Federation
Russian Federation
I. N. Anisimova
Всероссийский институт генетических ресурсов растений имени Н.И. Вавилова
Russian Federation
Russian Federation
References
1. Анащенко А.В., Дука М.В. Фенотипическое проявление генов Rf у подсолнечника и некоторые данные о природе растений с частично восстановленной фертильностью // Науч. техн. бюлл. ВИР. – 1985. – Вып. 154. – С. 3-7.
2. Воронова О.Н., Толстая Т.Т., Рожкова В.Т., Гаврилова В.А. Определение фертильности пыльцы у ряда диких многолетних видов и образцов подсолнечника из коллекции, произрастающей на Кубанской станции ВИР // Труды по прикладной ботанике, генетике и селекции. 2011. – Т. 167. – С. 145-158.
3. Воронова О.Н., Гаврилова В.А. Количественный и качественный анализ пыльцы подсолнечника (Helianthus L.) и его использование в селекционной работе // Труды по прикладной ботанике, генетике и селекции. 2019. – Т. 180. – № 1. – С. 95-104. DOI: 10.30901/2227-8834-2019-1-95-104
4. Хохлов C.C., Зайцева М.И., Куприянов П.Г. Выявление апомиктичных форм во Флоре апомиктичных растений СССР. Программа, методика, результаты. – Изд-во Саратовского университета. – 1978. – 224 c.
5. Anisimova I.N., Alpatieva N.V., Voronova O.N., Gavrilova V.A., Karabitsina Yu.I., Kuznetsova E.B., Radchenko E.E. A recombination suppressed region of sunflower (Helianthus annuus L.) linkage group 13 covers restoration of fertility (Rf1) and downy mildew resistance (Pl) gene clusters // Russian Journal of Genetics. – 2023. − Vol. 59. − No 5. − P. 453-465. DOI: 10.1134/S1022795423050022
6. Dimitrijevic A., Horn R. Sunflower hybrid breeding: from markers to genomic selection // Frontiers in Plant Science. – 2018. − Vol. 17. − № 8. DOI: 10.3389/fpls.2017.02238
7. Gautam R., Shukla P., Kirti P.B. Male sterility in plants: an overview of advancements from natural CMS to genetically manipulated systems for hybrid seed production // Theoretical and Applied Genetics. – 2023. − Vol. 136. − No 9. DOI: 10.1007/s00122-023-04444-5. PMID: 37606708
8. Karabitsina Y.I., Gavrilova V.A., Alpatieva N.V., Kuznetsova T.B., Anisimova I.N. Peculiarities of inheritance of pollen fertility restoration trait in sunflower with cytoplasmic male sterility // Russian Journal of Genetics. – 2019. − Vol. 55. − No 11. − P. 1375-382. DOI: 10.1134/S1022795419110073
9. Kaul M.L.H. Genic male sterility. In: Male sterility in higher plants. Monographs on Theoretical Applied Genetics. − Springer, Berlin, Heidelberg Berlin, 1988. − Vol. 10. DOI: 10.1007/978-3-642-83139-3
10. Kinman M.L. New developments in the USDA and state experiment station sunflower breeding programs. In: Proceedings of the 4th International Sunflower Conference, Memphis, TN, USA. – 1970. − Р. 181-183.
11. Leclerq P. Une sterilite cytoplasmique chez le tournesol // Annales de l'Amélioration des Plantes. 1969. – Vol. 19. – № 2. – P. 99-106.
12. Kováčik A., Sýkorová O. Comparison of the germinability of sunflower pollen in vivo and the results of tests for pollen viability // Sbornik UVTIZ, Genetika a Slechteni. – 1979. – Vol. 14. – No 2. – P. 81-86
13. Radwan O., Bouzidi M.F., Nicolas P. Mouzeyar S. Development of PCR markers for the Pl5/Pl8 locus for resistance to Plasmopara halstedii in sunflower, Helianthus annuus L. from complete CC-NBS-LRR sequences // Theoretical and Applied Genetics. – 2004. – Vol. 109. – P. 176-185. DOI: 10.1007/s00122-004-1613-0
14. Rieseberg L.H., Blackman B.K. Speciation genes in plants // Annals of Botany. – 2010. – Vol. 106. – No 3. – P. 439-455.
Review
For citations:
Voronova O.N., Karabitsyna Yu.I., Alpatieva N.V., Gavrilova V.A., Anisimova I.N. Phenomenon of cytoplasmic male sterility in sunflower and possible causes of reduced seed setting in F2 hybrids. Bulletin of the State Nikitsky Botanical Gardens. 2024;1(153):97-106. (In Russ.)
Views:
49
Email this article 