Разработка и применение инновационного олигонуклеотидного инсектицида явол-11 для контроля Ceroplastes japonicus green (подотряд sternorrhyncha)

Изобретенные в 2008 г. контактные олигонуклеотидные инсектициды (олинциды или ДНК-инсектициды) на основе платформы контактно вводимой антисмысловой ДНК-биотехнологии (КВАДб) были существенно улучшены и переосмыслены. КВАДб (или метод "генетической застёжки-молнии") объединяет молекулярную генетику, биоинформатику и синтез нуклеиновых кислот in vitro. Главным сдвигом парадигмы стала демонстрация того, что немодифицированная антисмысловая ДНК может действовать как контактный инсектицид. Ключевые прорывы включали идентификацию удобных целевых генов (генов рРНК), механизма действия (ДНК-сдерживание) и обнаружение насекомых-вредителей из подотряда грудохоботных (Sternorrhyncha) с высокой восприимчивостью к олинцидам. Данный класс инсектицидов нового поколения создает возможности для разработки инсектицидов, предназначенных для отдельных популяций насекомых-вредителей. В данной работе, проведенной на личинках Ceroplastes japonicus Green в открытом грунте, показан высокий потенциал использования олинцида ЯВОЛ-11 (5′-CGACCGACGAA-3′) в борьбе с ложнощитовкой и их потенциал в качестве замены неселективным фосфоорганическим инсектицидам.

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