Development and application of innovative oligonucleotide insecticide YAVOL-11 for control of Ceroplastes japonicas Green (suborder Sternorrhyncha)
Abstract
Contact oligonucleotide insecticides (olinscides or DNA insecticides) based on the contact unmodified antisense DNA biotechnology (CUADb) platform, invented in 2008, have been significantly improved and rethought. CUADb (or the "genetic zipper" method) combines molecular genetics, bioinformatics and in vitro nucleic acid synthesis. A major paradigm shift was the demonstration that unmodified antisense DNA can act as a contact insecticide. Key breakthroughs included the identification of convenient target genes (rRNA genes), the mechanism of action (DNA containment) and the detection of sternorrhynchans insect pests highly susceptible to olinscides. This class of new generation insecticides creates opportunities for the development of insecticides intended for individual populations of insect pests. In this work, conducted on Ceroplastes japonicus Green larvae in open ground, the high potential of using olinscide YAVOL-11 (5'-CGACCGACGAA-3') in the fight against false scale insects and their potential as a replacement for non-selective organophosphorus insecticides is demonstrated.
About the Authors
Yu. V. Plugatar
Никитский ботанический сад – Национальный научный центр РАН
Russian Federation
Russian Federation
N. V. Gal’chinsky
ФГАОУ ВО «КФУ им. В.И. Вернадского»
Russian Federation
Russian Federation
E. V. Yatskova
Никитский ботанический сад – Национальный научный центр РАН
Russian Federation
Russian Federation
A. K. Sharmagiy
Никитский ботанический сад – Национальный научный центр РАН
Russian Federation
Russian Federation
V. V. Oberemok
Никитский ботанический сад – Национальный научный центр РАН; ФГАОУ ВО «КФУ им. В.И. Вернадского»
Russian Federation
Russian Federation
References
1. Araújo M.F., Castanheira E.M.S., Sousa S.F. The Buzz on Insecticides: A Review of Uses, Molecular Structures, Targets, Ad-verse Effects, and Alternatives // Molecules. – 2023. – Vol. 28. – P. 3641. DOI: 10.3390/molecules28083641
2. Bachellerie J.P., Cavaille J., Huttenhofer A. The expanding snoRNA world // Biochimie. – 2002. – Vol. 84. – P. 775-790. DOI: 10.1016/S0300-9084(02)01402-5
3. Bachellerie J.P., Cavaille J., Huttenhofer A. The expanding snoRNA world // Biochimie. – 2002. – Vol. 84. – P. 775-790. DOI: 10.1016/S0300-9084(02)01402-5
4. Belikova A., Zarytova V., Grineva N. Synthesis of ribonucleosides and diribonucleoside phosphates containing 2-chloroethylamine and nitrogen mustard residues // Tetrahedron Lett. – 1967. – Vol. 37. – P. 3557-3562. DOI: 10.1016/S0040-4039(01)89794-X
5. Boukouvala M.C., Kavallieratos N.G., Skourti A., Pons X., Alonso C.L., Eizaguirre M., Fernandez E.B., Solera E.D., Fita S., Bohinc T., Trdan S., Agrafioti P., Athanassiou C.G. Lymantria dispar (L.) (Lepidoptera: Erebidae): current status of biology, ecology, and management in europe with notes from North America // Insects. – 2022. – Vol. 13. – P. 854. DOI: 10.3390/insects13090854
6. De Schutter K., Taning C.N.T., Van Daele L., Van Damme E.J.M., Dubruel P., Smagghe G. RNAi-based biocontrol products: market status, regulatory aspects, and risk assessment // Front Insect Sci. – 2022. – Vol. 1. – P. 818037. DOI: 10.3389/finsc.2021.818037
7. Dias N., Stein C.A. Antisense oligonucleotides: basic concepts and mechanisms // Mol Cancer Ther. – 2002. – Vol. 1. – P. 347-55.
8. Donis-Keller H. Site specific enzymatic cleavage of RNA // Nucleic Acids Res. – 1979. – Vol. 7. – P. 179-92. DOI: 10.1093/nar/7.1.179
9. Egli M., Manoharan M. Chemistry, structure and function of approved oligonucleotide therapeutics // Nucleic Acids Res. – 2023. – Vol. 51. – P. 2529-73. DOI: 10.1093/nar/gkad067
10. Gal’chinsky N.V., Yatskova E.V., Novikov I.A., Sharmagiy A.K., Plugatar Y.V., Oberemok V.V. Mixed insect pest populations of Diaspididae species under control of oligonucleotide insecticides: 3′-end nucleotide matters // Pesticide Biochem Physiol. – 2024. – Vol. 200. – P. 105838. DOI: 10.1016/j.pestbp.2024.105838
11. Geary R.S., Henry S.P., Grillone L.R. Fomivirsen: clinical pharmacology and potential drug interactions // Clin Pharmacokinet. – 2002. – Vol. 41. – P. 255-60. DOI: 10.2165/00003088-200241040-00002
12. Guo Y., Ji N., Bai L., Ma J., Li Z. Aphid viruses: a brief view of a long history // Front Insect Sci. – 2022. – Vol. 2. – P. 846716. DOI: 10.3389/finsc.2022.846716
13. Keyel P.A. DNases in health and disease // Dev Biol. – 2017. – Vol. 429. – P. 1-11. DOI: 10.1016/j.ydbio.2017.06.028
14. Knorre D.G. Chemical instruments in modern biology (on the example of antisense effects on genetic structures) // Soros Educ J. – 1998. – Vol. 12. – P. 25-31.
15. Krieg A.M. CpG motifs in bacterial DNA and their immune effects // Annual Review of Immunology. – 2002. – Vol. 20. – P. 709-760. DOI: 10.1146/annurev.immunol.20.100301.064842
16. Kumar H., Verma S., Behera R.K., Chandel A., Sharma M., Sagar, D. DNA Insecticide: An Emerging Crop Protection Technology // Indian Journal of Entomology. – 2024. – Vol. 87(4). – P. 1-8. DOI: 10.55446/IJE.2024.2160
17. Lazarević-Pašti T., Milanković V., Tasić T., Petrović S., Leskovac A. With or Without You?-A Critical Review on Pesticides in Food // Foods. – 2025. – Vol. 14. – P. 1128. DOI: 10.3390/foods14071128
18. Lin M., Hu X., Chang S., Chang Y., Bian W., Hu R., Wang J., Zhu Q., Qiu J. Advances of antisense oligonucleotide technology in the treatment of hereditary neurodegenerative diseases // Evid Based Complement Alternat Med. – 2021. – Vol. 2021. – P. 6678422. DOI: 10.1155/2021/6678422
19. Oberemok V.V. Method of Elimination of Phyllophagous Insects from Order Lepidoptera // Ukraine Patent. – 2008. – No. 36445.
20. Oberemok V., Gal’chinsky N., Novikov I., Sharmagiy A., Yatskova E., Laikova E., Plugatar Y. Ribosomal RNA-Specific Antisense DNA and Double-Stranded DNA Trigger rRNA Biogenesis and Insecticidal Effects on the Insect Pest Coccus hesperidum // Int. J. Mol. Sci. – 2025. – Vol. 26. – P. 7530. DOI: 10.3390/ijms26157530
21. Oberemok V.V., Gal’chinsky N.V., Useinov R.Z., Novikov I.A., Puzanova Y.V., Filatov R.I., Kouakou N.J., Kouame K.F., Kra K.D., Laikova K.V. Four most pathogenic superfamilies of insect pests of suborder sternorrhyncha: invisible superplunderers of plant vitality // Insects. – 2023. – Vol. 14. – P. 462. DOI: 10.3390/insects14050462
22. Oberemok V., Laikova K., Ali J., Gal’chinsky N. CUADb-based oligonucleotide insecticides (contact unmodified antisense DNA biotechnology) vs. RNA biocontrols (double stranded RNA technology): newly born fraternal twins in plant protection // biorXiv. – 2024. DOI: 10.1101/2024.03.13.584797
23. Oberemok V.V., Laikova K.V., Andreeva O.A., Gal’chinsky N.V. Biodegradation of insecticides: oligonucleotide insecticides and double-stranded RNA biocontrols paving the way for eco-innovation // Front Environ Sci. – 2024. – Vol. 12. – P. 1430170. DOI: 10.3389/fenvs.2024.1430170
24. Oberemok V.V., Laikova K.V., Andreeva O.A., Gal’chinsky N.V. Oligonucleotide insecticides and RNA-based insecticides: 16 years of experience in contact using of the next generation pest control agents // J Plant Dis Prot. – 2024. – Vol. 131. – P. 1837-1852. DOI: 10.1007/s41348-024-00949-3
25. Oberemok V.V., Laikova K.V., Gal’chinsky NV. Contact unmodified antisense DNA (CUAD) biotechnology: list of pest species successfully targeted by oligonucleotide insecticides // Front Agron. – 2024. – Vol. 6. – P. 1415314. DOI: 10.3389/fagro.2024.1415314
26. Oberemok V.V., Laikova K.V., Gal’chinsky N.V., Useinov R.Z., Novikov I.A., Temirova Z.Z., Shumskykh M.N., Krasnodubets A.M., Repetskaya A.I., Dyadichev V.V.,Fomochkina I.I., Bessalova E.Y., Makalish T.P., Gninenko Y.I., Kubyshkin A.V. DNA insecticide developed from the Lymantria dispar 5.8S ribosomal RNA gene provides a novel biotechnology for plant protection // Sci Rep. – 2019. Vol. 9(6197). – P. 1-10. DOI: 10.1038/s41598-019-42688-8
27. Oberemok V.V., Laikova K.V., Useinov R.Z., Gal’chinsky N.V., Novikov I.A., Yurchenko K.A., Volkov M.E., Gorlov M.V., Brailko V.A., Plugatar Y.V. Insecticidal activity of three 10–12 nucleotides long antisense sequences from 5.8S ribosomal RNA gene of gypsy moth Lymantria dispar L. against its larvae // J Plant Prot Res. – 2019. – Vol. 59. – P. 561-564. DOI: 10.24425/jppr.2019.131271
28. Oberemok V.V., Laikova K.V., Zaitsev A.S., Gushchin V.A., Skorokhod O.A. The RING for gypsy moth control: Topical application of fragment of its nuclear polyhedrosis virus anti-apoptosis gene as insecticide // Pestic Biochem Physiol. – 2016. – Vol. 131. – P. 32-39. DOI: 10.1016/j.pestbp.2016.01.006
29. Oberemok V.V., Laikova K.V., Zaitsev A.S., Shumskykh M.N., Kasich I.N., Gal’chinsky N.V., Bekirova V.V., Makarov V.V., Agranovsky A.A., Gushchin V.A., Zubarev I.V., Kubyshkin A.V., Fomochkina I.I., Gorlov M.V., Skorokhod O.A. Molecular alliance of Lymantria dispar multiple nucleopolyhedrovirus and a short unmodified antisense oligonucleotide of its anti-apoptotic IAP-3 gene: A novel approach for gypsy moth control // Int J Mol Sci. – 2017. – Vol. 18. – P. 2446. DOI: 10.3390/ijms18112446
30. Oberemok V.V., Novikov I.A., Yatskova E.V., Bilyk A.I., Sharmagiy A.K., Gal’chinsky N.V. Potent and selective ‘genetic zipper’ method for DNA-programmable plant protection: innovative oligonucleotide insecticides against Trioza alacris Flor // Chem Biol Technol Agric. – 2024. – Vol. 11(144). – P. 1-8. DOI: 10.1186/s40538-024-00668-9
31. Oberemok V.V., Skorokhod O.A. Single-stranded DNA fragments of insect-specific nuclear polyhedrosis virus act as selective DNA insecticides for gypsy moth control // Pestic Biochem Physiol. – 2014 – Vol. 113. – P. 1-7. DOI: 10.1016/j.pestbp.2014.05.005
32. Oberemok V.V., Useinov R.Z., Skorokhod O.A., Gal’chinsky N.V., Novikov I.A., Makalish T.P., Yatskova E.V., Sharmagiy A.K., Golovkin I.O., Gninenko Y.I., Puzanova Y.V., Andreeva O.A., Alieva E.E., Eken E., Laikova K.V., Plugatar Y.V. Oligonucleotide insecticides for green agriculture: regulatory role of contact DNA in plant–insect interactions // Int J Mol Sci. – 2022. – Vol. 23. – P. 15681. DOI: 10.3390/ijms232415681
33. Oirdi M., Yaseen M., Farwa U., Raza M.A., Farhan M., Sandhu Z.A., Ali F., Khan H.S., Nahvi I. Crops and people: the dangers and potential benefits of pesticides // Cogent Food Agric. – 2024. – Vol. 10. DOI: 10.1080/23311932.2024.2334096
34. Patil V., Jangra S., Ghosh A. Advances in antisense oligo technology for sustainable crop protection. Crit Rev Plant Sci. – 2024. – P. 1-23. DOI: 10.1080/07352689.2024.2394001
35. Peng Y., Wang K., Fu W., Sheng C., Han Z. Biochemical comparison of dsRNA degrading nucleases in four different insects // Front Physiol. – 2018. – Vol. 9. – P. 624. DOI: 10.3389/fphys.2018.00624
36. Schernthaner J.P., Milne R.E., Kaplan H. Characterization of a novel insect digestive DNase with a highly alkaline pH optimum // Insect Biochem Mol Biol. – 2002. – Vol. 32. – P. 255-263. DOI: 10.1016/S0965-1748(01)00084-4
37. Skendžić S., Zovko M., Živković I.P., Lešić V., Lemić D. The Impact of Climate Change on Agricultural Insect Pests // Insects. – 2021. Vol. 12. – P. 440. DOI: 10.3390/insects12050440
38. Thakur S., Sinhari A., Jain P., Jadhav H.R. A perspective on oligonucleotide therapy: Approaches to patient customization // Front Pharmacol. – 2022. – Vol. 13. – P. 1006304. DOI: 10.3389/fphar.2022.1006304
39. Wang X.W., Blanc S. Insect transmission of plant single-stranded DNA viruses // Annu Rev Entomol. – 2021. – Vol. 66. – P. 389-405. DOI: 10.1146/annurev-ento-060920-094531
40. Wang Y., Zhang H., Li H., Miao X. Second-generation sequencing supply an effective way to screen RNAi targets in large scale for potential application in pest insect control // PloS One. – 2011. – Vol. 6. – P. e18644. DOI: 10.1371/journal.pone.0018644
41. Will C.L., Luhrmann R. Spliceosomal UsnRNP biogenesis, structure and function // Curr Opin Cell Biol. – 2001. – Vol. 13. – P. 290-301. DOI: 10.1016/S0955-0674(00)00211-8
42. Wu W., Shan H.W., Li J.M., Zhang C.X., Chen J.P., Mao Q. Roles of bacterial symbionts in transmission of plant virus by hemipteran vectors // Front Microbiol. – 2022. – Vol. 13. – P. 805352. DOI: 10.3389/fmicb.2022.805352
43. Zamecnik P.C., Stephenson M.L. Inhibition of Rous sarcoma virus replication and cell transformation by a specific oligodeoxynucleotide // Proc Natl Acad Sci USA. – 1978. – Vol. 75. – P. 280-284. DOI: 10.1073/pnas.75.1.280
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Plugatar Yu.V., Gal’chinsky N.V., Yatskova E.V., Sharmagiy A.K., Oberemok V.V. Development and application of innovative oligonucleotide insecticide YAVOL-11 for control of Ceroplastes japonicas Green (suborder Sternorrhyncha). Bulletin of the State Nikitsky Botanical Gardens. 2025;(156):7-17. (In Russ.)
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