Secretion of biologically active substances by glandular trichomes of different morphological types in some Pelargonium (Geraniaceae) species
Abstract
A series of histochemical tests were carried out for the qualitative determination of secondary metabolites in glandular trichomes (GTs) of leaves and flower elements in three Pelargonium (Geraniaceae) species: P. odoratissimum, P. exstipulatum and P. vitifolium. GTs of five morphological types are found in the studied species. The secreted substances may contain phenols, alkaloids, terpenes and acidic polysaccharides. The set of detected secondary compounds differs in different types of trichomes. In addition, there are species-specific staining features in histochemical reactions in GTs of all types. The most intense secretion and a greater variety of components are found in all trichomes of the 1st type. At the same time, trichomes of the 2nd and the 4th types have differences in the composition of secretions due to their localization on different organs. The synthesized substances can be accumulated in the cytoplasm of the GTs or be excreted into the subcuticular cavity.
About the Authors
U. A. Ryabukha
ФГБУН Ботанический институт им. В.Л. Комарова РАН
Russian Federation
Russian Federation
L. E. Muravnik
ФГБУН Ботанический институт им. В.Л. Комарова РАН
Russian Federation
Russian Federation
References
1. Рябуха У.А., Пожванов Г.А., Муравник Л.Е. Секреторные структуры листьев и цветков у представителей семейства Geraniaceae // Материалы VI (ХIV) Международной ботанической конференции молодых учёных в Санкт-Петербурге ‒ Санкт-Петербург: БИН РАН, 2025. ‒ C. 180-181.
2. Рябуха У.А., Муравник Л.Е. Железистые трихомы листьев и цветков у трёх видов Pelargonium (Geraniaceae) // Ботанический журнал. ‒ 2024. ‒ T. 109, № 10. ‒ C. 1010-1030. DOI: 10.31857/S0006813624100058
3. Ботаническая гистохимия: Пер. с англ. / Дженсен У. ‒ М.: Мир, 1965. ‒ 372 с.
4. Appezzato-da-Glória B., Da Costa F.B., da Silva V.C., Gobbo-Neto L., Rehder V.L.G., Hayashi A.H. Glandular trichomes on aerial and underground organs in Chrysolaena species (Vernonieae – Asteraceae): structure, ultrastructure and chemical composition // Flora Morphology, Distribution, Functional Ecology of Plants. ‒ 2012. ‒ Vol. 207(12). ‒ P. 878-887.
5. Ćavar S., Maksimović M. Antioxidant activity of essential oil and aqueous extract of Pelargonium graveolens L’Her // Food Control. ‒ 2012. ‒ Vol. 23(1). ‒ P. 263-267. DOI: 10.1016/j.foodcont.2011.07.031
6. David R., Carde J. Histochimie-coloration differentielle des inclusions lipidiques et terpeniques des pseudophylles du pin maritime au moyen du reactif NADI // Comptes Rendus Hebdomadaires Des Seances De L Academie Des Sciences. ‒ 1964. ‒ Vol. 258(4). ‒ P. 1338-1340.
7. Furr M., Mahlberg P.G. Histochemical analyses of laticifers and glandular trichomes in Cannabis sativa // Journal of Natural Products. ‒ 1981. ‒ Vol. 44(2). ‒ P. 153-159.
8. Guerrini A., Rossi D., Paganetto G., Tognolini M., Muzzoli M., Romagnoli C., Antognoni F., Vertuani S., Medici A., Bruni A. Chemical characterization (GC/MS and NMR fingerprinting) and bioactivities of south‐African Pelargonium capitatum (L.) L'her.(Geraniaceae) essential oil // Chemistry & biodiversity. ‒ 2011. ‒ Vol. 8(4). ‒ P. 624-642. DOI: 10.1002/cbdv.201000045
9. Ko K.N., Lee K.W., Lee S.E., Kim E.S. Development and ultrastructure of glandular trichomes in Pelargonium x fragrans ‘Mabel Grey’ (Geraniaceae) // Journal of Plant Biology. ‒ 2007. ‒ Vol. 50(3). ‒ P. 362-368. DOI: 10.1007/BF03030668
10. Levin D.A. The role of trichomes in plant defense // The quarterly review of biology. ‒ 1973. ‒ Vol. 48(1), Part 1. ‒ P. 3-15.
11. Muravnik L.E. The Structural Peculiarities of the Leaf Glandular Trichomes: AReview // Plant Cell and Tissue Differentiation and Secondary Metabolites / Ramawat K., Ekiert H., Goyal S. (eds). – Cham: Springer, 2020. ‒ P. 1-35. DOI: 10.1007/978-3-030-11253-0_3-1
12. Muravnik L.E., Mosina A.A., Zaporozhets N.L., Bhattacharya R., Saha S., Ghissing U., Mitra A. Glandular trichomes of the flowers and leaves in Millingtonia hortensis (Bignoniaceae) // Planta. ‒ 2021. ‒ Vol. 253(1). – ID 13. DOI: 10.1007/s00425-020-03541-9
13. Tahir S.S., Liz-Balchin M., Husain S.Z., Rajput M.T.M. SEM studies of trichomes types in representative species of the sect. Polyactium and sect. Ligularia in the genus Pelargonium L'Hérit., (Geraniaceae) // Pakistan Journal of Botany. ‒ 1994. ‒ Vol. 26. ‒ P. 397-407.
14. Tsai T., Diggle P., Frye H., Jones C. Contrasting lengths of Pelargonium floral nectar tubes result from late differences in rate and duration of growth // Annals of botany. ‒ 2017. ‒ Vol. 121. – P. 549-560 DOI: 10.1093/aob/mcx171
15. Yohana R., Chisulumi P.S., Kidima W., Tahghighi A., Maleki-Ravasan N., Kweka E.J. Anti-mosquito properties of Pelargonium roseum (Geraniaceae) and Juniperus virginiana (Cupressaceae) essential oils against dominant malaria vectors in Africa // Malaria Journal. ‒ 2022. ‒ Vol. 21(1). ‒ P. 1-15. DOI: 10.1186/s12936-022-04220-8
Review
For citations:
Ryabukha U.A., Muravnik L.E. Secretion of biologically active substances by glandular trichomes of different morphological types in some Pelargonium (Geraniaceae) species. Bulletin of the State Nikitsky Botanical Gardens. 2025;(156):18-24. (In Russ.)
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