Spectral studies of the cell surface to detect azulenes in plants
https://doi.org/10.25684/0513-1634-2023-147-90-95
Abstract
Possible application of spectral research methods for the determination of azulenes on the surface of plant cells promising as pharmacological raw materials has been considered. Leaf surfaces with blue or silver color in Eucalyptus cinerea F. Muell. ex Benth., Hippophae rhamnoides L., needles and cypress cones (berry-cones) Juniperus excelsa M. Bieb. and Juniperus × pfitzeriana (Späth) P.A. Schmidt 'Blue Cloud' were investigated. The peaks of 580-620 nm and 660-666 nm characteristic of azulenes and chlorophyll, respectively, have been recorded in the absorption spectra of leaves and cypress cones using a microspectrofluorimeter/ microspectrophotometer. 10 minute extracts from the surface of intact cells with 100% acetone or 95% ethanol have been analyzed to confirm the presence of azulenes in the samples. The content of azulenes in such extracts was in mg/g of wet weight in the leaves of Eucalyptus cinerea 12.5 ± 0.02 and Hippophae rhamnoides – 1.0 ± 0.1, and in the needles/cypress cones of juniper Juniperus excelsa – 1.76/1.46 ± 0.3/0.2 and Juniperus × pfitzeriana 'Blue Cloud' 0.55/2.96/ ± 0.01/0.3, accordingly. All the studied woody plants can be sources of azulenes.
About the Authors
V. V. Roschina
Институт биофизики клетки, Пущинский биологический центр РАН
Russian Federation
Russian Federation
G. A. Soltani
ФГБУ «Сочинский национальный парк»
Russian Federation
Russian Federation
V. V. Fateryga
3Карадагская научная станция им. Т.И. Вяземского – природный заповедник РАН – филиал Института биологии южных морей им. А.О. Ковалевского РАН
Russian Federation
Russian Federation
N. K. Prizova
Институт биофизики клетки, Пущинский биологический центр РАН
Russian Federation
Russian Federation
L. M. Khaibulaeva
Институт биофизики клетки, Пущинский биологический центр РАН
Russian Federation
Russian Federation
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Review
For citations:
Roschina V.V., Soltani G.A., Fateryga V.V., Prizova N.K., Khaibulaeva L.M. Spectral studies of the cell surface to detect azulenes in plants. Bulletin of the State Nikitsky Botanical Gardens. 2023;(147):90-95. (In Russ.) https://doi.org/10.25684/0513-1634-2023-147-90-95
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