Evaluation of various methods of grafting miniature roses on non-rooted cuttings in order to obtain stem forms

Studies  have  been  conducted  on  the  possibility  of  accelerating  the  production  of  stem  seedlings  of  5 cultivars of  miniature roses.  Two species  were used as rootstock – Rosa canina L. and R. multiflora Thunb. in the form of unrooted cuttings of three different lengths. According to the results of the research, it was revealed that cuttings without prior rooting are more promising for the graft component. They are cut from the species R. multiflora. When comparing the rootability of this species of R. canina, they revealed a high percentage of root formation. It was found that cuttings with the longest length of 40 cm show the best results in all variants of the study.  The  obtained  data  on  the  grafting  of  shoots  in  all  the  studied  rose  cultivars,  which  were  carried  out  on unrooted cuttings of the species R. multiflora, turned out to be promising. The effect on the process of splicing the  rootstock  with  the  graft  was  largely  influenced  by  the  method  of  conducting  the  grafting  operation.  Such  a method  of  inoculation  as  improved  copulation  in  the  blood  showed  the  best  results  in  all  the  studied  variants. The possibility of accelerated production of stem seedlings of rose cultivars from the miniature group has been confirmed by simultaneous rooting of the rootstock and splicing of the scion with the rootstock in closed ground conditions.

1. Бондорина И.А. Основные принципы повышения декоративных свойств древесных растений методами прививки. – Дисс. … канд. биол. наук. – М., 2000. – 110 с.

2. Васильева О.Ю., Амброс Е.В., Козлова М.В. Адаптивный потенциал подвоя Rosa canina L., полученного in vitro, в условиях Юга Западной Сибири // Сельскохозяйственная биология. – 2022. – Т. 57, № 3. – С. 579-590. DOI: 10.15389/agrobiology.2022.3.579rus

3. Доспехов Б.А. Методика полевого опыта (с основами статистической обработки результатов исследований). – М.: Агропромиздат, 1985. – 351 с.

4. Кръстев М.Т. Прививка как метод размножения и улучшения декоративности древесных растений // Проблемы рекреационных растений. – Чебоксары, 1984. – С. 111-115.

5. Кръстев М.Т., Бондорина И.А., Протас С.А. Биологические основы прививки древесных растений при интродукции. – М.: Товарищество научных изданий КМК, 2014. – 164 с.

6. Михайлов Н.Л. Выращивание штамбовых подвоев для роз. – М.: Б.и., 1958. – 20 с.

7. Окунева И.Б. Особенности вегетативного размножения сортовой сирени. – Дисс. … канд. биол. наук. – М., 1988. – 155 с.

8. Assunção M., Tedesco S., Fevereiro P. Molecular aspects of grafting in woody plants // Annual Plant Reviews Online. – 2021. – Vol. 4(1). – P. 87-126. DOI: 10.1002/9781119312994.apr0751

9. Balfagón D., Terán F., Reis de Oliveira T., Santa-Catarina C., Aurelio Gómez-Cadenas A. Citrus rootstocks modify scion antioxidant system under drought and heat stress combination // Plant Cell Reports. – 2021. – Vol. 41. – P. 593-602. DOI: 10.1007/s00299-021-02744-y

10. Baron D., Amaro A., Pina A., Ferreira G. An overview of grafting re-establishment in woody fruit species // Scientia Horticulturae. – 2019. – Vol. 243. – P. 84-91. DOI: 10.1016/j.scienta.2018.08.012

11. Castellano-Hinojosa A., Meyering B., Nuzzo A., Strauss S., Albrecht U. Effect of plant biostimulants on root and plant health and the rhizosphere microbiome of citrus trees in huanglongbing-endemic conditions // Trees. – 2021. – Vol. 35(5). – P. 15251539. DOI: 10.1007/s00468-021-02133-8

12. Davies F., Fann Y.-S. New propagation practices show promise for field-grown roses // American Nurseryman. – 1983. – Vol. 157(3). – P. 73-77.

13. Li W., Chen S., Liu Y., Wang L., Jiang J., Zhao S., Fang W., Chen F., Guan Z. Long-distance transport RNAs between rootstocks and scions and graft hybridization // Planta. – 2022. – Vol. 255(5). – P. 96. DOI: 10.1007/s00425-022-03863-w

14. Monder M., Niedzielski, M., Wolinski K. Effect of phenological stage and rooting enhancers on physiological parameters in stem cuttings in the process of rhizogenesis of Rosa × alba ‘Maiden’s Blush’ // Agriculture. – 2020. – Vol. 10(11). – P. 1-18. DOI: 10.3390/agriculture10110572

15. Ohkawa K. Cutting grafts as a means to propagate greenhouse roses // Science Horticulture. – 1980. – Vol. 13(2). – P. 91-199.

16. Padró M.D.A., Caboni E., Morin K.A.S., Mercado M.A.M., Olalde-Portugal V. Effect of Bacillus subtilis on antioxidant enzyme activities in tomato grafting // Peer J. – 2021. – Vol. 9. – P. 10984. DOI: 10.7717/peerj.10984

17. Qi W., Zhang C., Wang W., Cao Z., Li S., Li H., Zhu W., Huang Y., Bao M., He Y., Zheng R. Transcriptome analysis of different heat stress responses between self-root grafting line and heterogeneous grafting line in rose // Horticultural Plant Journal. – 2021. – Vol. 7(3). – P. 243-255. DOI: 10.1016/j.hpj.2021.03.004

18. Solgi M., Taghizadeh M., Bagheri H. Response of black mulberry onto white mulberry rootstock to stenting (cutting-grafting) techniques and IBA concentrations // Ornamental Horticulture. – 2022. – Vol. 28(1). – P. 78-84. DOI: 10.1590/2447-536X.V28I1.2413

19. Sun J., Hu R., Lv F., Yang Y., Tang Z., Zheng G., Li J., Tian H., Xu Y., Li. S. Comparative transcriptome analysis reveals stem secondary growth of grafted Rosa rugosa ‘Rosea’ scion and R. multiflora ‘Innermis’ rootstock // Genes. – 2020. – Vol. 11(2). – P. 228. DOI: 10.3390/genes11020228

20. Vahdati K., Sarikhani S., Mohammad Mehdi Arab M.M., Leslie C.A., Dandekar A.M., Aletà N., Bielsa B., Gradziel T.M., Montesinos Á.I., Rubio-Cabetas M.J., Sideli G.M., Serdar U., Akyüz B., Beccaro G.L., Donno D., Rovira M., Ferguson L., Akbari M., Sheikhi A., Adriana F. Sestras A.F., Kafkas S., Paizila A., Roozban M.R., Kaur A., Panta S., Zhang L., Sestras R.E., Mehlenbacher S.A. Advances in rootstock breeding of nut trees: Objectives and strategies // Plants. – 2021. – Vol. 10(11). – P. 2234. DOI: 10.3390/plants10112234

21. Van de Pol РА., Brenkelaar А. Stenting of roses; a method for quick propagation by simultaneously cutting and grafting // Scientia Horticulturae. – 1982. – Vol. 17. – P. 187-196.

22. Yuan H., Tai P., Gustave W., Xue F., Sun L. Grafting as a mitigation strategy to reduce root-to-shoot cadmium translocation in plants of Solanaceae family // Journal of Cleaner Production. – 2021. – Vol. 319. – P. 128708. DOI: 10.1016/j.jclepro.2021.128708

23. Zhang X., Feng Y., Khan K., Ullah N., Li Z., Zaheer S., Zhou R., Zhang Z. Quantitative proteomics-based analysis reveals molecular mechanisms of chilling tolerance in grafted cotton seedlings // Agronomy. – 2022. – Vol. 12(5). – P. 1152. DOI: 10.3390/agronomy12051