Influence of the composition of the culture media on the development of sporofites Osmunda regalis L. in vitro

Abstract

One of the important stages for the propagation of the rare endangered fern Osmunda regalis L. through spores is the regeneration of sporophytes from gametophytes at in vitro culture. The article shows that the effective formation of O. regalis sporophytes occurred earlier (after 60 days) and in a larger percentage (51.6%) on a culture medium with a lower salt content % MS, and the exclusion of ammonium nitrate and vitamins from the medium is significantly enhanced the growth of sporophytes, compared with other variants of media and control.