Marker-assisted analysis of strawberry hybrid seedlings according to anthracnose resistance (Rca2 gene)
https://doi.org/10.25684/0513-1634-2024-150-58-62
Abstract
Anthracnose fruit rot (C. acutatum) is a dangerous strawberry disease. One of the ways to control C. acutatum in strawberry plantations is the cultivation of cultivars with genetic resistance to this pathogen. Among the genetic factors of strawberry anthracnose resistance is the Rca2 gene, which determines resistance to C. acutatum isolates of the pathogenicity group 2. Using the diagnostic DNA marker STS-Rca2_240, molecular screening of strawberry hybrid forms according to the Rca2 anthracnose fruit rot resistance gene was carried out. Promising strawberry hybrids characterized by a heterozygous combination of alleles of the Rca2 gene were identified: 2/1-24, 2/1-34 (‘Quicky’ × ‘Olympia’), 3/9-5 (‘Florence’ × ‘Faith’), 3/12-2, 3/12-9 (‘Alisa’ × ‘Quicky’), 4/7-10, 4/7-19, 4/7-20 (‘Asia’ × ‘Aprica’), and 5/2-26 and 5/2-32 (‘San Andreas’ × ‘Monterey’).
About the Authors
A. S. LyzhinRussian Federation
I. V. Lukyanchuk
Russian Federation
References
1. Кузнецова А.А., Дудченко И.П., Копина М.Б. Антракноз земляники, классические и современные методы диагностики // Современные подходы и методы в защите растений. – 2018. – С. 70-73.
2. Лыжин, А.С., Лукъянчук И.В., Жбанова Е.В. Полиморфизм сортов земляники (Fragaria × ananassa) по гену устойчивости к антракнозу Rca2 // Труды по прикладной ботанике, генетике и селекции, 2019. – Т. 180, № 1. – С. 73-77. DOI: 10.30901/2227-8834-2019-1-73-77.
3. Лыжин А.С., Лукъянчук И.В. Молекулярный скрининг маркера гена устойчивости к антракнозной чёрной гнили (ген Rca2) генетической коллекции земляники // Дальневосточный аграрный вестник. – 2022. – Т. 16, № 3. – C. 12-19. DOI: 10.22450/199996837_2022_3_12
4. Лыжин А.С., Лукъянчук И.В. Анализ сортов земляники садовой по устойчивости к антракнозу с использованием диагностических ДНК-маркеров // Аграрная Россия. – 2022. – № 9. – С. 16-20. DOI: 10.30906/1999-5636-2022-9-16-20
5. Холод Н.А., Кащиц Ю.П., Добренков Е.А., Семёнова Л.Г. Оценка устойчивости сортов земляники садовой к антракнозной черной гнили в южном регионе // Плодоводство и виноградарство юга России. – 2018. – № 51(3). – С. 140-148. DOI: 10.30679/2219-5335-2018-3-51-140-148.
6. Ahmed M.F.A., El-Fiki I.A.I. Effect of biological control of root rot diseases of strawberry using Trichoderma spp. // Middle East Journal of Applied Sciences. – 2017. – Vol. 7(3). – P. 482-492.
7. Lerceteau-Kohler E., Roudeillac P., Markocic M., Guérin G., Praud K., Denoyes-Rothan B. The use of molecular markers for durable resistance breeding in the cultivated strawberry (Fragaria × ananassa) // Acta Hort. – 2002. – Vol. 567(2). – P. 615-618. DOI: 10.17660/ActaHortic.2002.567.132
8. Lerceteau-Kohler E., Guerin G., Denoyes-Rothan B. Identification of SCAR markers linked to Rca2 anthracnose resistance gene and their assessment in strawberry germplasm // Theor Appl Genet. – 2005. – Vol. 111. – P. 862-870. DOI: 10.1007/s00122-005-0008-1
9. Luk'yanchuk I.V., Lyzhin A.S., Kozlova I.I. Analysis of strawberry genetic collection (Fragaria L.) for Rca2 and Rpf1 genes with molecular markers // Vavilov Journal of Genetics and Breeding. – 2018. – Vol. 22(7). – P. 795-799. DOI: 10.18699/VJ18.423
10. Salinas N., Fan Z., Peres N., Lee S., Whitaker V.M. FaRCa1 confers moderat resistance to the root necrosis form of strawberry anthracnose caused by Colletotrichum acutatum // HortScience. – 2020. – Vol. 55(5). – P. 693-698. DOI: 10.21273/HORTSCI14807-20
11. Jacobs R.L., Adhikari T.B., Pattison J., Yencho G.C., Fernandez G.E., Louws F.J. Assessing rate-reducing foliar resistance to anthracnose crown rot and fruit rot in strawberry // Plant disease. – 2020. – Vol. 104(2). – P. 398-407. DOI: 10.1094/PDIS-04-19-0687-RE
12. Karimi K., Arzanlou M., Pertot I. Weeds as potential inoculum reservoir for Colletotrichum nymphaeae causing strawberry anthracnose in Iran and Rep-PCR fingerprinting as useful marker to differentiate C. acutatum complex on strawberry // Frontiers in microbiology. – 2019. – Vol. 10. – P. 129. DOI: 10.3389/fmicb.2019.00129
13. Sturzeanu M., Ciuca M., Cristina D., Turcu A.G. Use of RAPD and SCAR markers for identification of strawberry genotypes with red stele resistance genes Rpf1 and fruit rot resistance genes Rca2 in the hybrid progenies // IX International Strawberry Symposium. – 2021. – Vol. 1309. – P. 93-100. DOI: 10.17660/ActaHortic.2021.1309.15
Review
For citations:
Lyzhin A.S., Lukyanchuk I.V. Marker-assisted analysis of strawberry hybrid seedlings according to anthracnose resistance (Rca2 gene). Bulletin of the State Nikitsky Botanical Gardens. 2024;(150):58-62. (In Russ.) https://doi.org/10.25684/0513-1634-2024-150-58-62