Prospects of using DNA insecticide eriola-11 to control the number of woolly apple aphids Eriosoma lanigerum Hausmann, 1802

Eriosoma lanigerum (Hausmann, 1802) (woolly apple aphid) is one of the most common pests of fruit crops around the world, native to North America. It primarily affects representatives of the Rosaceae family, causing significant damage to them: it weakens vital functions, provokes the development of deformations and a greater likelihood of the spread of mold fungi, including increasing the risk of penetration of putrefactive bacteria into plant tissue. To combat insect pests, chemical insecticides are still used today, and natural enemies of woolly apple aphids are also used. However, if the former cause significant harm to the environment as a whole, negatively affecting biodiversity, then the latter do little to cope with the decrease in the number of E. lanigerum. The goal of our research was to develop a DNA insecticide based on mitochondrial rRNA to control the number of woolly apple aphids Eriosoma lanigerum (Hausmann, 1802). This article examines the effectiveness of the developed DNA insecticide Eriola-11 based on the degradation of the target 16S ribosomal RNA. This “genetic zipper” method has many positive features, such as safety for non-target organisms, biodegradability and high efficiency. During the study, single and double treatment of woolly apple aphid with the DNA insecticide Eriola-11 was carried out, as well as recording the number of dead individuals. Treatment with DNA insecticide against adult E. lanigerum showed positive results. The biological effectiveness of the insecticide was 68.0 ± 0.85% after a single treatment, and 85.3 ± 1.52% after a double treatment on the 14th day.

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