Determination of biologically active substances in extracts of (Angelica Archangelica L.) by NMR spectroscopy
Abstract
In modern pharmacopoeial analysis, there is an increasing demand for high-precision qualitative and quantitative data on plant raw materials used in pharmaceutical production, mainly due to strict quality control and safety standards. In addition, raw materials of the same species can vary significantly in the composition of secondary metabolites. Currently, in the field of phytochemical analysis, conventional high-performance liquid chromatography with various detection options or spectrophotometry in the visible and ultraviolet range are commonly used for qualitative and quantitative determination of substances. However, most of these methods have significant disadvantages such as long sample preparation time and the need for authentic standard samples [1, 2]. Nuclear magnetic resonance (NMR) spectroscopy is devoid of these limitations and therefore appears to be a perspective method for the identification and quantification of bioactive substances of plant origin. In this work, a targeted metabolomic analysis of Angelica archangelica L. by NMR spectroscopy was made, as a result of which 23 metabolites were detected and quantified in the water extract, and preliminary studies were carried out to develop a methodology for the identification and quantification of ostol in DMSO-d6 extract of Angelica archangelica L. roots by 1H NMR spectroscopy.
About the Authors
A. V. Sheremeta
Российский университет дружбы народов
Russian Federation
Russian Federation
V. G. Vasiliev
Российский университет дружбы народов
Russian Federation
Russian Federation
V. A. Ivlev
Российский университет дружбы народов
Russian Federation
Russian Federation
A. A. Murtazina
Российский университет дружбы народов
Russian Federation
Russian Federation
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Review
For citations:
Sheremeta A.V., Vasiliev V.G., Ivlev V.A., Murtazina A.A. Determination of biologically active substances in extracts of (Angelica Archangelica L.) by NMR spectroscopy. Bulletin of the State Nikitsky Botanical Gardens. 2025;1(155):132-140. (In Russ.)
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