FLORA AND VEGETATION
The phytocenotic and biomorphological features of Atraphaxis daghestanica, an endemic species of the Eastem Caucasus, hsted in the Red Data Book of the Repubhc of Dagestan (2009) in the Untsukul region of Dagestan (42 ° 36'21 "N, 46 ° 58'09" Е, 590 m above sea level) have been studied. The cenopopulation is represented mainly Ьу middle-aged generative plants, covers an area of 1 О hectares and has а population of about 10,000 specimens. The results of one-way analysis of variance showed that а significant intergroup difference was expressed for all the registered signs of an annual shoot.
The results of assessing of the spatiotemporal relationship and interannual dynamics the of regional ecosystems productivity of the Crimean Peninsula with hydmthennal conditions аге presented. Landsat space images from 1979 to 2017 аге used as the research material. The resolution of each image is 30х30 m The NDVI index was used to account for the productivity factor of ecosystems in the study. The ArcGIS 10 image analysis module is used to determine this index On the basis of the rank correlation coefficient, several clearly defined intervals are determined, which, in tum, allow for the selected time period to identify several quite clearly defined periods in determining the influence ofhydrothennal states of regional ecosystems on the fonnation of the value of their biological productivity. For the period from 1979 to 2017, four periods characterized Ьу different degrees of spatiotemporal correlation between productivity of regional ecosystems and their heat and moisture supply are identified. The first period is characterized Ьу relatively staЫe and sufficiently high values of spatial correlation between values of regional ecosystems productivity and indicators of hydrothennal states. The second period is represented Ьу much less staЫe indicators and fluctuations of spatial relationship in dynamics from year to year. The third period is associated with stabihzation of spatial relationship indicators and their increase to the level of the fIГSt period, while the relationship of productivity with temperatures is more staЫe in time. The fourth period, is characterized Ьу violation of staЫe dynamics in considered indicators, their fluctuations towards decrease of spatial relationship degree with possiЫe sharp jumps in case of especially dry and hot seasonal states, which, in general, correlates rather tightly with the shift of seasonal states. These periods are quite clearly connected with the changes in the prevailing circulation processes. The role of different circulation groups in the interannual dynamics of the spatial-temporal relationship of the characteristics under consideration is shown. It is noted that from 1957 up to 1985, the increase in the duration of the northem circulation had aЬsolute dominance. The barrier in 1985 coincides quite clearly with the change of the lst period of the present analysis. This clearly provided stabihty of the spatial relationship indicators in the lst period and some divergence in the 2nd period after the circulation type change. The 2nd period of the meridional Southem circulation epoch coincides with the lst period of staЫe relationship between heat and moisture availabihty indicators and values of productivity of regional ecosystems. The period from 1970 to 1997 was characterized Ьу southem cyclones exiting from the sea, which led to а decrease in air temperature in the Crimea on average per year, and to unstaЫe correlations with the productivity of ecosystems. From 1998 to the present, with the dominance of the meridional Northem circulation and the minimum duration of the Zonal circulation, there is а decrease in the duration of the meridional Southem circulation, which causes а sufficiently long period of staЫe relationships between productivity and the main climatic indicators in the 3rd period of this analysis. Decrease in mean annual temperature in 1981-1997 and its sharp increase in 1998-2013 clearly enough influence on the change of hnks with these parameters in pmductivity of regional ecosystems. The increase in air temperature in the period 1998-2013 is associated with the growth of the anticyclonic regime.
Three associations of the class Carpino-Fagetea Jakucs et Passarge 1968 were described as а result of the classification of forest communities of the Colchis Lowland (Westem Transcaucasia) using the Brown Blanquet method. Association Vaccinio arctostaphilli-Carpinetum betuli ass. nova prov. was included in the order Lathyro-Carpinetalia Passarge 1981, alliance Crataego-Carpinion Passarge 1981. Associations Truello thunbergii-Alnetum barbatae ass. nova prov. and Carici remotae-Pterocmyetum pterocarpae ass. nova prov. were included in the order Rhododendro ponticae-Fagetalia orientalis Quezel et al. 1992, alliance Alnion barbatae Quezel et al. 1992. The results of the DCA ordination and the interpretation of the leading axes demonstrated three well-distinguished ecological and floristic types of deciduous forests (ofthe association rank) forming the ecological series according to the leading ecological factors - the degree of humidity of habitats and the degree of drainage of substrates. Twenty-three plant species - thermophilous Tertiary rehcs were found in all three associations. Of these, eight evergreen species play а significant phytocoenotic role in the formation of the understory in forest communities.The pecuharities of the Tertiary rehc speciesdistributions in the floristic compositions of described associations are discussed.
he article summarizes the hterary and proprietary data on the moss flora of Ayu-Dag State Nature Reserve. The features of the taxonomic stmcture of bryophytes in this territory are considered. The brioflora of Ayu-Dag mount is represented Ьу 98 species belonging to 66 genera, 41 families, 12 orders and 4 classes: of which 17 аге liverworts and 81 leafy moss. Identified taxonomic features аге characteristic of southem floras. А comparison with the Crimean bryoflora is given, showing а high percentage of species diversity of the study агеа, The high sozological value of bryoflora has been estabhshed - 56.1% of the species are included in the regional Red Books of the Russian Federation, and 11 species аге hsted in the Red Book of the RepuЫic of the Crimea, which сопбппз the high scientific importance and environmental value of the territory of the Ayu-Dag reserve.
The results of the ecological and floristic classification of some mderal communities of the city of Nalchik are presented. The community of Cichorium intybus (Artemisietea vulgaris) that consist of two classes of mderal vegetation, Artemisietea vulgaris and Stellarietea mediae, and the class of meadow vegetation Molinio-Arrhenatheretea, is described. The association Conyzo canadensis-Lactucetum serriolae Lohmeyer in Oberdorfer 1957 is described. Communities of associations, including those under the name Erigeronto Lactucetum serriolae Lohmeyer in Oberdorfer 1957, are noted not only in settlements, but аво in other biotopes in Russia and abroad.
AGROECOLOGY
Southem Federal University, Academy ofBiology and Biotechnology, Rostov-on-Don, Russia. А study of the enzyme activity of soils in two sites of the Abraus Peninsula in the coastal part of the Utrish reserve was conducted. Sites differ in the period of post-fire recovery. The long-term significant influence of fires on the enzyme activity (catalase, invertase, peroxidase, urease) of brown subtropical soils (Chromic Cambisols) is shown. The activity of various enzymes of soils is reduced compared to the soils of the control sites. The degree of inactivation of enzymes depends on the type of enzyme and the time of post-pyrogenic recovery of ecosystems.
PLANT BIOCHEMISTRY
The results of ascorbic acid and phenohc compounds accumulation dynamics in the buds of the genus Ficus species and cultivars with different degree offrost resistance, growing under the conditions of the Southem Coast of the Crimea, аге presented. It was found that the frost resistance of the genus Ficus studied genotypes depends on the degree of flavonols and ascorbic acid accumulation and does not depend оп the total concentration of phenohc compounds. The participation of ascorbic acid and phenohc substances in the implementation of defense mechanisms of the genus Ficus studied genotypes under low-temperature stress is assumed.
Despite the high interest of researchers in the study of organic acids in various plant objects, there is no information on the composition of organic acids for many officinal plant species. Such species include а plant known for а long time in medical practice - Blue Jacob's Ladder (Polemonium caeruleum L.), belonging to the family of Polemoniaceae.
Samples of grass and rhizomes with roots Blue Jacob's Ladder were used as the studied plant material and were harvested in the Altai Territory and purchased from а private supplier. Blue Jacob's Ladder grass was collected during flowering, underground organs - in autumn, in the first year of the plant's life. The quantitative total organic acid content in terrns of malic acid was carried out Ьу the titrimetric method according to phaпnacopoeial monograph.2.5.0093.18 State Pharmacopoeia of the RF XIV ed. "Rowan ordinary fmit". The experimental study of the quahtative composition of organic acids and the assessment of their quantitative content in the grass of the studied objects was carried out using the electrophoretic method (Кареl, St. Petersburg, Russia). А high content of organic acids in grass and rhizomes with roots Blue Jacob's Ladder was estabhshed Ьу the phaпnacopoeial method. Ву the electrophoretic method the profile and quantitative content of organic acids of Blue Jacob's Ladder was studied for the fIГSt time, а comparative research of their content in grass and rhizomes with roots was carried out. The difference between grass and root-peel with roots of the studied plant is shown, both in component and quantitative composition of organic acids.
PLANT BIOTECHNOLOGY
An in vitro micropropagation protocol has been developed for garden chrysanthemum cultivars Mokroe Serebro, Predrassvetnyiy Ayu-Dag, Eldorado, and Ermitage of the NВG's breeding. 10-20 mg/1 of ribavirin promoted the primary explants cleaning up. А high frequency of regeneration of adventitious buds and microshoots was observed in cultivars Eldorado and Ermitage (21.0 and 23.5 microshoots/explant) cultured on MS medium with 0.75 mg/1 kinetin and Mokroe Serebro - on MS medium with 0.75 mg/1 ВАР (24.5 microshoots/explant). Spontaneous root formation facilitated 100% rooting of microshoots.
ESSENTIAL OIL AND MEDICINAL PLANTS
This article presents the results of studies on the identification of the diagnostic traitsof Actinidia arguta leaves and the content of the prevailing groups of biologically active compounds (ВАС). The objective of this work is to estabhsh diagnostic traits of Actinidia arguta (Siebold & Zucc.) Planch. Ех Miq. leaves for the identification of raw materials and predominant ВАС with pharmacological activity. Using macroscopic and microscopic methods of phannacognostic analysis, in accordance with the requirements of the State Pharmacopoeia of the XIV edition, extemal and microscopic traits of Actinidia arguta have been estaЫished. The results of the studies allow reliaЫe identification of leavesof Actinidia arguta, according to characteristic extemal and microdiagnostictraits. Phytochemical analysis in raw materials deteпnined the presence of ВАС: polysaccharides, ascorbic acid, amino acids, flavonoids, tannins.
It ,,vas found that the inhalation of vapors of abies sibirica essential oil (Abies siЬirica Ledeb.) at а 3 has а pronounced positive effect on the psychoemotional state of the elderly. А decrease in the level of anxiety and depression and an improvement in self-esteem of the general state, well being, mood, stress, ,vorking efficiency, vigor, and attentiveness are observed for all the studied exposure durations (10, 20, 30 minutes). The effect of abies sibirica essential oil on mental performance is ambiguous. Abies sibirica essential oil in а dosage of 1 mg/m concentration of 1 mg/m3 in elderly people (60-90 years) in all durations of exposure, it has а pronounced euphoric effect, helps to overcome psychological difficulties and optimize the emotional state.
ORNAMENTAL HORTICULTURE
The article contains а hst of introduced specimens of the genus Spiraea L. in the Polar-Alpine Botanical Garden-Institute located abovethe Arctic Circle. The collection fund ofthe Polar-Alpine Botanical Garden-Institute contains 51 Spiraea specimens belonging to 12 species, 1 subspecies, 3 varieties, 4 cultivars and 2 hybrids. Most of the introduced accessions аге of seed origin. The age of the collection spireas is from 4 to 79 years. The article gives а comprehensive assessment of the viability and decorativeness of introduced plants of the genus Spiraea in the Kola Subarctic. Evaluated the degree of hgnification of the annual shoot, winter hardiness, retention of gmwth fonn, shoot-fonning ability, height gain, abihty to generative development, methods of reproduction in culture, crown architectonics, duration and abundance of flowering, color and size of flowers and inflorescences, attractiveness of fmits, color range of autumn leaf color, leafing duration, plant агата, damage, correlation between viability, decorative effect and the period of being in the experiment. Observations for 2000-2020 were used in the work. А verage score of winter hardiness of introduced S. betuhfoha, S. chamaedryfolia, S. nipponica, S. media 1-3. S. japonica, S. japonica 'Norroboth', S. х bumalda, S. х bumalda 'Shraederii', S. х cinerea 'Grefsheim', S. х rosalba аге frozen to the snow cover and soil level. Winter-hardy species retain their vital fonn, weak-hardy ones are аЫе to restore their growth foпn Most of the studied samples Ыооm and bear fmit not annually, reproduction is possiЫe with artificial sowing, using cuttings. The studied samples can Ье divided into groups: quite viaЫe and viaЫe high and medium decorative; less and less viaЬle high and medium decorative; non-viaЫe and absolutely non-viaЫe medium and low decorative. The most viaЫe and decorative under conditions of introduction to the Kola Peninsula are S. betuhfolia, S. chamaedryfoha, S. nipponica, S. media. S. japonica, S. japonica 'Norroboth', S. х bumalda, S. х bumalda 'Shraederii', S. х cinerea 'Grefsheim', S. х rosalba have low decorative qualities due to low viability.
The introduction study of Allium platycaule S. Wats. was cani.ed out: seasonal rhythm of growth and development, seed productivity, reproduction, the characteristic of age states of initial stages of ontogenesis and assessed the success and perspective of the species under the culture аге given. А. platycaule is а short-vegetative ephemeroid plant with early flowering. In seed reproduction, the generative state occurs at 6 year of vegetation. Flowering occurs in early June, the duration of flowering Ьу year is 12-17 days. The seeds mature in early July. The vegetation lasts about 2,5 months. From 15 to 30 flowers form in the inflorescence. The fmit formation of the umbrella varies from 26 to 89 % Ьу years. The real seed productivity of 1 umbrella averages 25,4 pieces of seeds. The fmit contains an average of 2 pieces of seeds. The efficiency coefficient of umbrella ranges from 8 to 40%. The potential opportunities аге realized Ьу an average of 200/о. The natural vegetative reproduction coefficient is 3,5. Weight of 1000 seeds - 4,9 g. Winter resistance is good, attacks afterreengineering were not observed. According to the assessment of introduction resistance, А. platycaule is а promising plant.
PLANT PROTECTION
The effect of microelement manganese top-dressing on resistance of soft wheat to powdery mildew № pathogen was studied. То study the effect of the element manganese on the development of the pathogen, wheat seedlings were fed with а 0,2% solution of КМn04. Infected plants not fed with the test solution served as control.
Observations on the intensity of germination of conidia of the powdery mildew pathogen on wheat leaves showed that the spomlation of conidia in the variant with feeding with 0,2% КМn04 solution was lower than in the control variant. We found that in the control variant without the use of а КМn04 solution, the colonies of powdery mildew fungus were characterized Ьу abundant spomlation, the formation of а halo was observed. In the experimental variant the growth and reproduction of conidia proceeded more slowly and halos were not noticeaЫe. In this study in the control variant ofthe experiment, the predominance of conidial chains in the colonies ofthe powdery mildew pathogen was noted in comparison with the variant with feeding.
Thus, it was found that feed solution of0,2% КМn04 leads to а decrease in the formation of appressoria and the number of visiЫe colonies of the powdery mildew pathogen on wheat leaves. The presented results confIГm that top-dressing with microelement manganese enhances resistance ofwheat against powdery mildew.